cat 4480 lr  (R&D Systems)

Journal: International journal of molecular sciences

Article Title: The Antimicrobial Peptide AMP-IBP5 Suppresses Dermatitis-like Lesions in a Mouse Model of Atopic Dermatitis through the Low-Density Lipoprotein Receptor-Related Protein-1 Receptor.

doi: 10.3390/ijms24065200

Figure Lengend Snippet: Figure 3. AMP-IBP5 regulates TJ barrier function in human keratinocytes via LRP1. Human primary keratinocytes were pretreated with 1 µg/mL recombinant human LRPAP protein (i.e., RAP) for 24 h and stimulated with 10 µM AMP-IBP5 for 48 h. (A) Immunofluorescence staining of the TJ- related proteins claudin-1, occludin, and ZO-1 upon stimulation with AMP-IBP5. The results of one representative experiment of three independent experiments are shown. Scale bar: 50 µm. (B) TER was measured after AMP-IBP5 treatment. The values obtained using stimulated and non- stimulated keratinocytes in the presence and absence of RAP were compared. (C) Immunoblot analysis using antibodies specific for phosphorylated and unphosphorylated aPKCζ and Rac1. ** p < 0.01, *** p < 0.001, # p < 0.05, ## p < 0.01. All data are representative of three independent experiments.

Article Snippet: Recombinant human and mouse LRPAP were obtained from R&D Systems.

Techniques: Recombinant, Staining, Western Blot

Journal: International journal of molecular sciences

Article Title: The Antimicrobial Peptide AMP-IBP5 Suppresses Dermatitis-like Lesions in a Mouse Model of Atopic Dermatitis through the Low-Density Lipoprotein Receptor-Related Protein-1 Receptor.

doi: 10.3390/ijms24065200

Figure Lengend Snippet: Figure 8. The restorative effect of AMP-IBP5 on skin barrier function is blocked by RAP injection in mice. (A) Representative images of H&E staining in AD mice co-injected with AMP-IBP5 and recombinant mouse LRPAP (i.e., RAP). Scale bar: 200 µm; n = 3/group. (B) TEWL in mouse ears on Day 19. (C) Representative immunofluorescence images (left) and quantitative analysis of the claudin-1 staining intensity in the mouse epidermis (right). Scale bar: 20 µm; n = 3 mice/group. (D) A biotin tracer was also used to assess TJ permeability. Tracer exclusion is indicated by the yellow arrowheads. Scale bar: 5 µm; n = 3 mice/group. The data are presented as the means ± SDs. * p < 0.05, **** p < 0.0001, ## p < 0.01, #### p < 0.0001, $$ p < 0.01, $$$$ p < 0.0001, % p < 0.05. All data are representative of three independent experiments.

Article Snippet: Recombinant human and mouse LRPAP were obtained from R&D Systems.

Techniques: Injection, Staining, Recombinant, Permeability

Journal: International journal of molecular sciences

Article Title: The Antimicrobial Peptide AMP-IBP5 Suppresses Dermatitis-like Lesions in a Mouse Model of Atopic Dermatitis through the Low-Density Lipoprotein Receptor-Related Protein-1 Receptor.

doi: 10.3390/ijms24065200

Figure Lengend Snippet: Figure 9. The suppressive effect of AM-IBP5 on inflammation is blocked by RAP injection in AD mice. (A) The mRNA levels of IL-4, IL-13, IL-31, IL-33, and TSLP and the total serum level of IgE in AD mice co-injected with AMP-IBP5 and recombinant mouse LRPAP (i.e., RAP) were measured. (B) Representative images of immunostaining of CD4+ T cells in AD mice (left) and the number of CD4+ T cells (right) in the stained sections. Scale bar: 50 µm; n = 3 mice/group. (C) Representative images of toluidine blue staining of mast cells, indicated by the black arrowheads (left) and the number of mast cells (right) in the stained sections. Scale bar: 50 µm; n = 3 mice/group. The data are presented as the means ± SDs. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ## p < 0.01, ### p < 0.001, #### p < 0.0001, $ p < 0.05, $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001. All data are representative of three independent experiments.

Article Snippet: Recombinant human and mouse LRPAP were obtained from R&D Systems.

Techniques: Injection, Recombinant, Immunostaining, Staining